ISSN : 2583-2646

Surfactant Extraction from Slaughterhouse Offals: Recovery, Characterization, and Biomedical Applications

ESP Journal of Engineering & Technology Advancements
© 2026 by ESP JETA
Volume 6  Issue 2
Year of Publication : 2026
Authors : Dr. Anandhu A Kumar, Dr V V Kulkarni, Joshika Panda, Kriti, Lavisha Gupta
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Citation:

Dr. Anandhu A Kumar, Dr V V Kulkarni, Joshika Panda, Kriti, Lavisha Gupta, 2026. Surfactant Extraction from Slaughterhouse Offals: Recovery, Characterization, and Biomedical Applications  Volume 6 Issue 2: 141-148.

Abstract:

The application of surfactants is diverse and extends into biomedical applications, industries, and food science owing to their capacity to lower surface and interfacial tensions. The increasing need for sustainability and superior performance attributes has led to growing interest in the design of natural or biosurfactants that are sourced from animal and microorganism sources. The inedible animal by-products like lungs, skins, and connective tissues can be effectively used to obtain value-added materials like phospholipids, collagen, gelatin, and its derivatives, which exhibit high surface activity.

References:

[1] N. A. Doran-Browne, R. J. Eckard, R. Behrendt and R. S. Kingwell, Nutrient density as a metric for greenhouse gas emissions. Clim Change. 129(2015) 73–87.

[2] R. Han, J. T. Shin, J. Kim, Y. S. Choi and Y. W. Kim, Edible insect food industry overview. Entomol Res. 47(2017) 141–151.

[3] W. Biel, E. Czerniawska-Piatkowska and A. Kowalczyk, Offal chemical composition from veal, beef, and lamb. Animals. 9(2019) 489.

[4] K. G. O. Bezerra, R. D. Rufino, J. M. Luna and L. A. Sarubbo, Saponins and microbial biosurfactants: potential raw materials for cosmetics formulation. Biotechnol Prog. 34(2018) 1482–1493.

[5] A. Singh, J. D. Van Hamme and O. P. Ward, Surfactants in biotechnology. Biotechnol Adv. 24(2006) 100–116.

[6] J. A. Shelford and G. Kamande, Feed additive surfactants. U.S. Patent. (2001).

[7] P. Mokrejs, F. Langmaier, M. Mladek, D. Janacova, K. Kolomaznik and V. Vasek, Collagen extraction from meat by-products. Waste Manag Res. 27(2009) 31–37.

[8] R. Ramanathan, Animal-derived surfactants. J Perinatol. 29(2009) S38–S43.

[9] M. Griese, Pulmonary surfactant in health and disease. Eur Respir J. 13(6) (1999) 1455–1476.

[10] R. A. Polin and W. A. Carlo, Surfactant replacement therapy. Pediatrics. 133(2014) 156–163.

[11] I. Kralova and J. Sjoblom, Surfactants used in food industry: a review. 2009.

[12] J. D. Brain and N. R. Frank, Recovery of free cells from rat lungs by repeated washings. J Appl Physiol. 25(1) (1968) 63–69.[13] Bligh, E.G. and Dyer, W.J. (1959) Can. J. Biochem. Physiol. 37. 911-917.[14] N. B. Smith, Lipid analysis methods. Lipids. 17(1982) 464–468.

[13] Bligh, E.G. and Dyer, W.J. (1959) Can. J. Biochem. Physiol. 37. 911-917.[14] N. B. Smith, Lipid analysis methods. Lipids. 17(1982) 464–468.

[14] N. B. Smith, Lipid analysis methods. Lipids. 17(1982) 464–468.

[15] J. C. Touchstone, J. C. Chen and K. M. Beaver, Thin-layer chromatography methods. Lipids. 15(1980) 61–62.

[16] G. Rouser, S. Fleischer and A. Yamamoto, Lipid separation techniques. Lipids. 5(1970) 494–496.[17] F. Possmayer, G. Duwe, M. Hahn and D. Buchnea, Lipid analysis. Can J Biochem. 55(1977) 609–617.

[17] F. Possmayer, G. Duwe, M. Hahn and D. Buchnea, Lipid analysis. Can J Biochem. 55(1977) 609–617.

[18] M. Kates, Techniques of lipidology. Amsterdam: Elsevier. (1972).

[19] L. M. G. Van Golde and L. L. M. Van Deenen, Phospholipid metabolism. Biochim Biophys Acta. 125(1966) 496–509.

[20] O. H. Lowry, N. J. Rosenbrough, A. L. Farr and R. J. Randall, Protein measurement method. J Biol Chem. 193(1951) 265–275.

[21] I. L. Metcalfe, G. E. Enhorning and F. Possmayer, Pulmonary surfactant studies. J Appl Physiol. 49(1980) 34–41.

[22] G. Enhorning, Pulsating bubble method for surfactant evaluation. J Appl Physiol. 43(1977) 198–203.

[23] B. Robertson, Surfactant substitution studies. Lung. 158(1980) 57–68.

[24] Lachmann, B., Tischer, A.B., Grossman, G. and Robertson, B., 1981. Lung compliance and alveolar expansion in the artificially ventilated premature newborn rabbit after maternal treatment with ambroxol. Respiration, 42(3), pp.209-216.

[25] T. Fujiwara, S. Chida, Y. Watabe, H. Maeta, T. Morita and T. Abe, Artificial surfactant therapy. Lancet. 1(1980) 55–59.

[26] J. A. Smyth, I. L. Metcalfe, P. Duffy et al., Clinical surfactant studies. Clin Invest Med. 4(1981) 43.

[27] R. I. Sanders, Lung development and surfactant. In: P. M. Farrell, editor. Lung Development. New York: Academic Press. (1982) 193–210.

[28] R. J. King and J. A. Clements, Surface active materials from dog lung. Am J Physiol. 223(3) (1972) 715–726.

[29] J. L. Harwood, R. Desai, P. Hext, R. Tetley and R. Richards, Lung surfactant composition. Biochim J. 151(1975) 707–714.

[30] A. Jobe, M. Ikegami, T. Glatz, Y. Yoshida, F. Diakomanolis and J. Padbury, Surfactant metabolism studies. J Clin Invest. 67(1981) 370–375.

[31] Y. Suzuki, E. Nakai and K. Ohkawa, Lipid composition studies. J Lipid Res. 23(1982) 53–61.

[32] K. Ohno, T. Akino and T. Fujiwara, Surfactant metabolism. Rev Perinat Med. 2(1978) 227–280.

[33] M. Oulton, T. R. Martin, G. T. Faulkner, D. Stinson and J. P. Johnson, Surfactant composition studies. Pediatr Res. 14(1980) 722–728.

[34] A. D. Bangham, C. J. Morley and M. C. Phillips, The physical properties of an effective lung surfactant. Biochim Biophys Acta. 573(3) (1979) 552–556.

[35] I. L. Metcalfe, R. Burgoyne and G. Enhorning, Surfactant function studies. Pediatr Res. 16(1982) 834–839.

[36] S. B. Ainsworth, M. W. Beresford, D. W. Milligan, N. J. Shawn, J. N. Matthews, A. C. Fenton et al., Pumactant and poractant alfa for treatment of respiratory distress syndrome in neonates born at 25–29 weeks’ gestation: a randomised trial. Lancet. 355(2000) 1387–1392.

[37] F. R. Moya, J. Gadzinowski, E. Bancalari et al., Lucinactant vs other surfactants. Pediatrics. 115(2005) 1018–1029.

[38] S. K. Sinha, T. Lacaze-Masmonteil, A. Soler et al., Lucinactant vs poractant alfa. Pediatrics. 115(2005) 1030–1038.

[39] F. Moya, S. Sinha, J. Gadzinowski et al., One-year follow-up of surfactant therapy. Pediatrics. 119(2007) e1361–e1370.

[40] W. A. Engle and Committee on Fetus and Newborn, Surfactant-replacement therapy for respiratory distress. Pediatrics. 121(2008) 419–432.

[41] P. A. Dargaville and J. M. Mills, Surfactant therapy for meconium aspiration syndrome. Drugs. 65(18) (2005) 2569–2591

[42] B. Chacko, Surfactants as feed additives in ruminants: a review. Int J Adv Res Biol Sci. 7(5) (2020) 8–17.

[43] L. H. Lin and K. M. Chen, Gelatin-derived surfactants. Colloids Surf A. 272(2006) 8–14.

[44] J. R. Wright, Surfactant clearance. Am J Physiol. 259(1990) L1–L12.

[45] J. R. Wright, Immunoregulatory functions of surfactant proteins. Nat Rev Immunol. 5(2005) 58–68.

[46] R. Veldhuizen and F. Possmayer, Surfactant metabolism. Subcell Biochem. 37(2004) 359–388.

[47] E. Putman, L. A. J. M. Creuwels, L. M. Van Golde et al., Pulmonary surfactant subtypes. Biochem J. 320(1996) 599–605.

[48] R. A. Veldhuizen, L. A. McCaig, T. Akino et al., Surfactant subfractions. Am J Respir Crit Care Med. 152(1995) 1867–1871.

[49] R. H. Notter, Lung surfactants: basic science and clinical applications. New York: Dekker. (2000).

[50] I. Frerking, A. Gunther, W. Seeger et al., Pulmonary surfactant: functions and abnormalities. Intensive Care Med. 27(2001) 1699–1717.

[51] E. P. Ingenito, R. Mora and L. Mark, Role of anionic phospholipids in surfactant. Am J Respir Crit Care Med. 161(2000) 831–838.

[52] U. Christmann, V. A. Buechner-Maxwell, S. G. Witonsky and R. D. Hite, Role of lung surfactant in respiratory disease. J Vet Intern Med. 23(2) (2009) 227–242.

[53] A. Tridente, L. De Martino and D. De Luca, Porcine vs bovine surfactant therapy. Respir Res. 20(2019) 28.

[54] N. Singh, K. L. Hawley and K. Viswanathan, Porcine vs bovine surfactants. Pediatrics. 128(2011) e1588–1595.

Keywords:

Surfactants, Animal by-products, Biosurfactants, Pulmonary surfactant, Gelatin derivatives, Sustainability